First Report of Groundnut Bud Necrosis Virus in Pergularia daemia (Asclepiadaceae) in India
نویسندگان
چکیده
منابع مشابه
Bud necrosis of groundnut (Arachis hypogaea) in India caused by tomato spotted wilt virus*
Chlorotic ringspots or chlorotic leaf specking, terminal bud necrosis, axillary shoot proliferation and severe stunting of groundnut (Arachis hypogaea) were shown to be caused by tomato spotted wilt virus (TSWV). Cowpea (Vigna unguiculata cv. C-152) was found to be a good assay host. TSWV remained infective in buffered sap of groundnut at a dilution of 10-2’5, after storage for 4 h at room temp...
متن کاملImmunodiagnosis of groundnut and watermelon bud necrosis viruses using polyclonal antiserum to recombinant nucleocapsid protein of Groundnut bud necrosis virus.
In vitro gene expression strategy was used for the production of polyclonal antiserum to the nucleocapsid protein (NP) of Groundnut bud necrosis virus (GBNV). The GBNV NP gene from cowpea isolate was cloned into 6x His-tagged UA cloning vector and expressed in Escherichia coli [M15] cells. The fusion protein was detected in insoluble fraction and was purified by using Ni-NTA agarose resin. The ...
متن کاملEffect of Temperature on Symptoms Expression and Viral RNA Accumulation in Groundnut Bud Necrosis Virus Infected Vigna unguiculata
Background: Groundnut bud necrosis virus (GBNV) (Tospovirus genus, Bunyaviridae family) infects the major crops of solanaceae, leguminosae and cucurbitaceae in India. Temperature is an important factor which influences the plant growth and development under diseased conditions.Objective: In the present study, we evaluated the effect of four different temp...
متن کاملDiuretic Potential of Whole Plant Extracts of Pergularia daemia (Forsk.)
The whole plant, Pergularia daemia (Family: Asclepediaceae), was extracted with 50% alcohol and a fresh batch of the plant material was successively extracted with petroleum ether, ethyl acetate and n-butanol to determine its diuretic activity. The diuretic activity of the different extracts at a dose of 400 mg/Kg was assessed orally in rats with furosemide as a standard drug using Lipschitzs t...
متن کاملPreliminary Phytochemical Screening of Asteracantha longifolia and Pergularia daemia
The aim of the study was to analyze the phytochemical constituents of two potential folklore medicinal plants such as Asteracantha longifolia and Pergularia daemia. Methanol and Aqueous extract of the dried leaves of these plants were collected and used for phytochemical analysis. The selected plants were found to contain alkaloids, phenolic compounds, tannins and flavonoids except for the abse...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 2018
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis-02-18-0358-pdn